南寧師范大學蘇煒&林陳想等：具有近紅外響應的卟啉自組裝納米光敏劑實現高效I型光動力和光熱治療
2025-05-22 15:09:46
作者：中國科學材料 來源：中國科學材料
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具有近紅外光響應的I型光敏劑對氧的依賴性較低，可實現有效的電子轉移，在缺氧微環境光動力治療中具有極大的應用潛力。
近日，南寧師范大學蘇煒教授、林陳想博士、廣西中醫藥大學李培源研究員和北京科技大學姜建壯教授等人在Science China Materials發表研究論文，通過5,10,15,20-四(4-氨基苯基)卟啉（TAPP）與N-芐氧羰基-L-亮氨酸（Cbz-Leu）的共組裝策略，成功構建了超分子納米光敏劑TAPP@Cbz-Leu。
本文要點
1) 在近紅外光（808 nm）照射下，由于TAPP與Cbz-Leu之間的非共價的緊密結合，可誘導光生電子從Cbz-Leu向三重態TAPP轉移，生成TAPP·-，因 此，TAPP@Cbz-Leu可以顯著生成O2·-。此外，TAPP@Cbz-Leu在808 nm近紅外光照射下具有優異的光熱性能。2) 體內實驗表明，TAPP@Cbz-Leu對MRSA感染的動物傷口模型具有良好的抗菌效果，并能有效促進傷口愈合。
本研究首次通過簡單共組裝策略實現了II型光敏劑向I型光敏劑的轉化，為開發新型近紅外響應的光療材料的構建提供了新思路。
Figure 1. Schematic illustration of the conversion of type II photosensitizer (TAPP) triggered by 660?nm light to type I photosensitizer (TAPP@Cbz-Leu) triggered by 808?nm NIR light.Figure 2. UV-vis absorption spectroscopy of (a) TAPP@Cbz-Leu with different TAPP and Cbz-Leu stoichiometric ratios, and (b) TAPP, Cbz-Leu, the mixed solution of TAPP and Cbz-Leu in DMSO, and TAPP@Cbz-Leu in water. (c) Fluorescence intensities of TAPP in DMSO and TAPP@Cbz-Leu in water. (d) TEM image of TAPP@Cbz-Leu. (e) Size distribution in water detected by DLS. (f) FT-IR spectra of TAPP, Cbz-Leu and TAPP@Cbz-Leu.
Figure 3. (a) ROS generation of TAPP@Cbz-Leu in water with irradiation, detected by DCFH. (b) ROS generation of TAPP, ICG and TAPP@Cbz-Leu in water with irradiation. (c) 1O2 production of TAPP@Cbz-Leu in water with irradiation detected using SOSG. (d) O2·- production of TAPP@Cbz-Leu in water with irradiation detected using DHE. (e) O2·- production of TAPP and TAPP@Cbz-Leu in water upon irradiation. (f) O2·- production of TAPP and TAPP@Cbz-Leu upon irradiation under normal and hypoxic conditions. (g) ESR spectra of O2·- trapped by DMPO in TAPP@Cbz-Leu with or without irradiation. (h) Energy levels of TAPP@Cbz-Leu and TAPP. (i) Nanosecond transient absorption spectra of TAPP and TAPP@Cbz-Leu dispersed in water (0.5?mM). All the irradiation is 808?nm, 0.5?W/cm2.
Figure 4. (a–c) Viability and (d) representative pictures of MRSA, S. aureus and E. coli following treatment with TAPP@Cbz-Leu. (e) Live/Dead staining of MRSA treated with TAPP@Cbz-Leu. (f) Detection of ROS generation of TAPP@Cbz-Leu via DCFH probe. (g) SEM images of S. aureus and E. coli with and without treatment with TAPP@Cbz-Leu. Error bars represent the mean ± SD (n ≥ 3). All the irradiation is 808?nm, 0.5?W/cm2.
Wei Su (蘇煒) received his BS and PhD degrees from Shandong University, and then he worked at North Carolina State University as a postdoc. He joined Nanning Normal University in 2008 and is currently a full professor. His work focuses on porphyrin and phthalocyanine materials and their applications in photocatalysis.
Lei Cen (岑磊) received his Master’s degree under the supervision of Prof. Wei Su and Dr. Chenxiang Lin at Nanning Normal University. His research interest mainly focuses on phototherapy antibacterial of porphyrin and phthalocyanine self-assembled nanomaterials.
Dong Yang (楊棟) is currently studying for a Master’s degree under the supervision of Prof. Wei Su and Dr. Chenxiang Lin at Nanning Normal University. His research focuses on preparation of functional hydrogels and antimicrobial phototherapy.
Chenxiang Lin (林陳想) received her BS degree from Guangxi Normal University in 2015 and PhD degree from University of Science and Technology Beijing in 2021. Then, she worked at Guangxi Key Laboratory of Natural Polymer Chemistry and Physics of Nanning Normal University as a lecturer. Her research focuses on the design and synthesis of porphyrin and phthalocyanine functional materials for phototherapy antibacterial and photocatalytic hydrogen production.
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